Traditionally, donor tissue for simple limbal epithelial transplantation (SLET) in patients with unilateral limbal stem cell deficiency (LSCD) is taken from the opposite normal eye. However, in cases of partial LSCD, clinically, assessment of the presence of healthy limbus in the same eye presents the possibility of taking donor tissue from the same eye. In this video, a 5-year-old girl with lime-powder injury underwent human amniotic membrane transplantation after removal of the lime particles. Later, she developed partial LSCD in the affected eye. Donor limbal tissue was taken from the same eye of the patient to perform SLET without recurrence and a stable ocular surface. This possibility should be kept in mind when assessing patients with LSCD.