Preparation of an intraocular tumor specimen. A, Transillumination shows blockage of light due to an intraocular tumor. B, The area of blockage is marked with a marking pencil. C, The opened eye shows the intraocular tumor that was demonstrated by transillumination. D, The paraffin-embedded eye shows the intraocular tumor. E, The hematoxylin-eosin (H&E)– stained section shows that the maximum extent of the tumor demonstrated by transillumination is in the center of the section, which includes the pupil and optic nerve.