Vitreous Biopsy Under Air

In this 1-Minute video, Drs. Kyle Kirkland and Ahmed Sallam demonstrate a technique for obtaining vitreous biopsy under air. Compared with dry vitrectomy, this method provides a much larger sample of undiluted vitreous without the risk of globe collapse; up to 2 ml of neat vitreous can be obtained. In the setup, it is important to eliminate any fluid from the vitrector and infusion line to avoid sample dilution. The presented case is of an 80-year-old patient with new-onset unilateral panuveitis and significant vitreous haze. Three sets of vitreous samples were sent out for bacterial and fungal cultures, herpes viruses and toxoplasma PCR and immunohistochemistry testing for lymphoma cells. 

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