DEC 01, 2009
This video demonstrates and compares the various techniques of donor dissection in Descemet membrane endothelial keratoplasty (DMEK). In an experimental, nonrandomized, comparative study, 40 human sclerocorneal rims were divided into 4 groups, 10 in each. Four different techniques were tried by a single surgeon. The techniques were (1) reverse big bubble, (2) blunt knife dissection, (3) trephination-scoring and peeling, and (4) trephination and descemetorrhexis. Success of complete separation and ease of dissection and reproducibility were most with technique 4 by trephination and descemetorrhexis.