Diagnostic Electron Microscopy

Historically, diagnostic electron microscopy (DEM) was used to indicate the cell of origin of a tumor of questionable differentiation rather than to distinguish between benign and malignant processes. DEM is now used in selected cases to complement immunopathologic studies when IHC is not sufficient for accurate diagnosis. However, DEM is less widely available and more expensive than IHC. The surgeon should consult with the pathologist before surgery to determine whether DEM could play a role in the study of a particular tissue specimen and to ensure that the tissue is fixed appropriately.

Excerpted from BCSC 2020-2021 series: Section 4 - Ophthalmic Pathology and Intraocular Tumors. For more information and to purchase the entire series, please visit https://www.aao.org/bcsc.