APR 01, 2020
This study assessed the tumor microenvironment of uveal melanoma using single cell analysis.
The authors analyzed the tumor microenvironment using droplet-based single-cell RNA sequencing (scRNA-seq). They obtained 59,915 uveal melanoma cells and non-neoplastic cells from 8 primary and 3 metastatic uveal melanomas.
There was a diversity of tumor-infiltrating immune cells in the tumor microenvironment, including CD8+ T cells. These cells predominantly expressed the checkpoint marker LAG3 rather than PD1 or CTLA4, which are current targets for checkpoint blockades. This could explain, in part, why currently available checkpoint inhibitors are minimally efficacious for treating metastatic uveal melanoma. It also provides a potential new target for checkpoint blockage.
The researchers analyzed a small number of primary tumors and metastases.
This study identified LAG3 as a potential target for immune checkpoint blockade in patients with high risk uveal melanoma.