This video covers various methods of IOL analysis that detect even the smallest traces of deposits or opacifications. Methods include light microscopy of stained samples, scanning electron microscopy, and x-ray spectroscopy. The IOLs show central opacifications due to deposits of calcium and phosphate below the anterior lens surface caused by exposure to air or gas bubbles during DSAEK and DMEK surgery. Experiments with new air-gas mixtures are performed to find a solution for this problem.